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Tein and RNA analysisThe sequence used for generating shRNA for nNOS was a double-stranded DNA of 21 nucleotides from 2281 to 2301 region of nNOS DNA. The first nucleotide of the target sequence started at `G', which is required by the RNA polymerase III promoter. We added a poly T termination signal for antisense oligonucleotide and an EcoRI restriction enzyme cutting site for cloning of the DNA
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Tein and RNA analysisThe sequence used for generating shRNA for nNOS was a double-stranded DNA of 21 nucleotides from 2281 to 2301 region of nNOS DNA. The first nucleotide of the target sequence started at `G', which is required by the RNA polymerase III promoter. We added a poly T termination signal for antisense oligonucleotide and an EcoRI restriction enzyme cutting site for cloning of the DNA